Basic Chromatography and Separation Technology in Analytical Labs
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Basic Chromatography and Separation Technology in Analytical Labs Course
Introduction:
The objective of this course is to offer comprehensive training in the fundamental techniques of gas chromatography (GC) and liquid chromatography (HPLC). It covers the essential components of chromatographic systems, operating principles, applications, calibration methods, setup procedures, preventative maintenance, troubleshooting techniques, quantitative methods, and failure modes for both GC and HPLC, supported by practical examples.
Additionally, the course delves into other important aspects such as optimizing column lengths, flow rates, temperatures, and pressures, providing the necessary theoretical information for each aspect. By equipping participants with these skills and knowledge, the course aims to enhance their expertise and understanding while enabling them to apply these techniques effectively.
Course Objectives:
- To impart participants with the fundamental techniques and knowledge of chromatography.
- To familiarize participants with the tools and techniques used in chromatography analyses, cause and effect diagrams, calibration, and standard analysis methods.
- To provide the skills, knowledge, and understanding of principles of chromatography analysis application.
- To use routine maintenance.
- To impart participants the operation technique.
- To train participants to know how to change and install all chromatography components.
- To be familiar with the latest analytical methods.
Who Should Attend?
The course is designed for chemists, lab technicians, chemical engineers, instrument engineers and lab supervisors/managers.
Course Outlines:
- Fundamental chromatography technique
- Chromatographic theory parameters
- Chromatography retention process
- Separation methods
- Application of chromatography
- Solid phase extraction method
- Derivatization method
- Standard operation method
- Successful and safe operating procedure
- Gas chromatography technique
- Carrier gas and pressure regulator system
- Split/splitless inlet system
- Cool on-column inlet and programmed temperature vaporization inlet
- Column configuration
- Detector configuration
- Maintenance and installation procedure for inject system, column and detectors
- Liquid chromatography technique
- Mobile phase solvent
- Pumping system
- Column and detector types
- Operation method of HPLC
- Overview of liquid chromatographic separation methods
- Application of liquid chromatography
- Ion chromatographic method
- Size-exclusion chromatographic method
- Supercritical fluid chromatography
- Instrumentation and operating variables
- Chromatography variables effect
- Techniques to minimize peak broadening
- Column condition and regeneration technique
- Maintenance and installation procedure for inject system, column, pump and detectors
- Troubleshooting and approaches to solve problems
- Quantitative methods: calibration methods, external and internal standards, outliers test, determination of analyte concentration, standard addition method, error in quantitative analysis, confidence limits, detection limit, repeatability, reproducibility, and method validation.